Media in bottles

Available in packages:

· bottle 100 ml, 10 pcs in a package

· bottle 200 ml, 10 pcs in a package

· bottle 500 ml, 6 pcs in a package

Storage and expiry date

Most bottled media should be stored at temperature of 6-25ºC. Expiry date for bottled media: 12 months from the date of production.

Attention! Use all the contents of the bottle once opened. Do not store the opened bottle!

Adding the supplements during preparation of media

Supplements should reach the room temperature before adding to media Prior to mixing the media should be cooled to the temperature of 45 -50ºC.

Preparation of bottled media for pouring onto Petri dishes

Ready bottled agar media may be melted in Koch apparatus at ca 80ºC, or in a microwave. If you are using a microwave:

1. Place the bottle filled with medium in a center of microwave.

2. Choose short time - usually 5-10 seconds (depending on the volume of medium).

3. Remove cautiously the slightly warmed bottle from the microwave and gently untight the cap to even out the pressure

4. Replace the bottle in a microwave and select the time (ca. 30 to 60 seconds). By that time most media will melt. If necessary, repeat the procedure for a dozen or so seconds, until melted. If you can see pieces of unmelted agar, just leave the bottle in a room temperature, it will melt completely by itself.

The warmed medium should be gradually cooled, until it reaches the optimum temperature for pouring (ca 45 – 50°C). Do not cool it rapidly.

According to the EN ISO 7218: 2007 “Microbiology of the food and animal feeding stuffs. General requirements and guidance for microbiological examinations” it is acceptable to melt culture media by means other then Koch apparatus, including microwaves.